Incubation:Article Title: Designing a method to test the efficacy of different sanitizers for use in rubber footbaths as applied to dairy manufacturing.
Article Snippet: This study designed an in vitro model to test the efficacies of footbath sanitizers for use in dairy processing plants.. Efficacies of selected sanitizers f or inactiv ation of mix ed-cocktail, dairy -rele v ant Gram-negativ e bacteria in model f ootbaths, in the presence of milk residues, w ere measured o v er 7 da y s against at tac hed and planktonic populations by plate counting and calculating log reductions achie v ed.. A simplified practical table rank ed the most to the least effective sanitizers.
Article Title: The applicability of fluorescent optotracers for in vitro and in vivo Staphylococcus aureus detection and quantification
Article Snippet: .. Bioluminescent S. aureus Xen36 (PerkinElmer Inc., Waltham, USA) was cultured from cryopreservative vials on Tryptone Soya Agar (TSA) (Oxoid Ltd., Basingstoke, UK, Fisher Scientific Int., Hampton, USA) plates with 200 μg/ml kanamycin (Sigma-Aldrich, St. Louis, USA) and incubated at 37 °C overnight. .. S. aureus ATCC 12600, S. epidermidis ATCC 35984 and E. coli ATCC 25922 were cultured on blood agar (Xebios Diagnostics Group, Düsseldorf, Germany) and incubated at 37 °C overnight.
Article Title: Cleaning compositions containing Dispersins III
Article Snippet: .. S. aureus was restreaked on Tryptone Soya Agar (TSA) (pH 7.3) (CM0131; Oxoid Ltd, Basingstoke, UK) and incubated for 1 day at 37° C. A single colony was inoculated into 10 mL of TSB and the culture was incubated for 16 hours at 37° C. with shaking (200 rpm). .. After propagation, the S. aureus culture was diluted (1:100) in fresh TSB+1% glucose (24563; Roquette Freres) and 2 mL aliquots were added to the wells of 12-well polystyrene flat-bottom microplates (3512; Costar, Corning Incorporated, Corning, N.Y., USA), in which round swatches (diameter 2 cm) of sterile polyester (WFK30A) had been placed.
Article Title: Cleaning compositions containing Dispersins III
Article Snippet: .. The Pseudomonas fluorescens strain was restreaked on Tryptone Soya Agar (TSA) (pH 7.3) (CM0131; Oxoid Ltd, Basingstoke, UK) and incubated for 1 day at 23° C. The strain was inoculated into 10 mL of TSB and the culture was incubated with shaking for 16 hours at 23° C. The overnight culture was diluted (1:100) in 200 ml M63 supplemented medium (15 mM (NH4)2SO4, 100 mM KH2PO4, 1.8 μM FeSO4, 1 mM MgSO4.7H20, 0.4% (w/v) glycerol, 0.2% (w/v) Casamino acids and 0.0001% (w/v) Thiamine) added to a Corning® CellBIND® 225 cm2 Angled Neck Cell Culture Flask with Vent Cap (Product #3293) and incubated statically for 5 days at 23° C. Each biofilm culture was subsequently transferred to four 50 ml falcon tubes (Corning #430820) and pelleted by centrifugation (10 min, 8000 g, 25° C.), and the supernatants were discarded completely. .. The residual pellets from each of the four falcons were resuspended in 0.450 ml 3M NaCl to extract the surface-associated EPS (extracellular polymeric substances) and pooled in one test tube (5 ml, Eppendorf #0030119401).
Cell Culture:Article Title: The applicability of fluorescent optotracers for in vitro and in vivo Staphylococcus aureus detection and quantification
Article Snippet: .. Bioluminescent S. aureus Xen36 (PerkinElmer Inc., Waltham, USA) was cultured from cryopreservative vials on Tryptone Soya Agar (TSA) (Oxoid Ltd., Basingstoke, UK, Fisher Scientific Int., Hampton, USA) plates with 200 μg/ml kanamycin (Sigma-Aldrich, St. Louis, USA) and incubated at 37 °C overnight. .. S. aureus ATCC 12600, S. epidermidis ATCC 35984 and E. coli ATCC 25922 were cultured on blood agar (Xebios Diagnostics Group, Düsseldorf, Germany) and incubated at 37 °C overnight.
Article Title: Cleaning compositions containing Dispersins III
Article Snippet: .. The Pseudomonas fluorescens strain was restreaked on Tryptone Soya Agar (TSA) (pH 7.3) (CM0131; Oxoid Ltd, Basingstoke, UK) and incubated for 1 day at 23° C. The strain was inoculated into 10 mL of TSB and the culture was incubated with shaking for 16 hours at 23° C. The overnight culture was diluted (1:100) in 200 ml M63 supplemented medium (15 mM (NH4)2SO4, 100 mM KH2PO4, 1.8 μM FeSO4, 1 mM MgSO4.7H20, 0.4% (w/v) glycerol, 0.2% (w/v) Casamino acids and 0.0001% (w/v) Thiamine) added to a Corning® CellBIND® 225 cm2 Angled Neck Cell Culture Flask with Vent Cap (Product #3293) and incubated statically for 5 days at 23° C. Each biofilm culture was subsequently transferred to four 50 ml falcon tubes (Corning #430820) and pelleted by centrifugation (10 min, 8000 g, 25° C.), and the supernatants were discarded completely. .. The residual pellets from each of the four falcons were resuspended in 0.450 ml 3M NaCl to extract the surface-associated EPS (extracellular polymeric substances) and pooled in one test tube (5 ml, Eppendorf #0030119401).
Isolation:Article Title: It Is Useless to Resist: Biofilms in Metalworking Fluid Systems
Article Snippet: .. Pseudomonas oleovorans , Pseudomonas aeruginosa, and Morganella morganii strains employed in all experiments were isolated from in-use MWF samples originating from Switzerland by standard heterotrophic plate count methods performed on Tryptone Soya Agar (TSA) prepared in-house (Oxoid CM0131, Thermo Fisher, Pratteln, Switzerland) at 40 g L −1 . ..
Article Title: It Is Useless to Resist: Biofilms in Metalworking Fluid Systems.
Article Snippet: .. Pseudomonas oleovorans, Pseudomonas aeruginosa, and Morganella morganii strains employed in all experiments were isolated from in-use MWF samples originating from Switzerland by standard heterotrophic plate count methods performed on Tryptone Soya Agar (TSA) prepared in-house (Oxoid CM0131, Thermo Fisher, Pratteln, Switzerland) at 40 g L−1. ..
Centrifugation:Article Title: Cleaning compositions containing Dispersins III
Article Snippet: .. The Pseudomonas fluorescens strain was restreaked on Tryptone Soya Agar (TSA) (pH 7.3) (CM0131; Oxoid Ltd, Basingstoke, UK) and incubated for 1 day at 23° C. The strain was inoculated into 10 mL of TSB and the culture was incubated with shaking for 16 hours at 23° C. The overnight culture was diluted (1:100) in 200 ml M63 supplemented medium (15 mM (NH4)2SO4, 100 mM KH2PO4, 1.8 μM FeSO4, 1 mM MgSO4.7H20, 0.4% (w/v) glycerol, 0.2% (w/v) Casamino acids and 0.0001% (w/v) Thiamine) added to a Corning® CellBIND® 225 cm2 Angled Neck Cell Culture Flask with Vent Cap (Product #3293) and incubated statically for 5 days at 23° C. Each biofilm culture was subsequently transferred to four 50 ml falcon tubes (Corning #430820) and pelleted by centrifugation (10 min, 8000 g, 25° C.), and the supernatants were discarded completely. .. The residual pellets from each of the four falcons were resuspended in 0.450 ml 3M NaCl to extract the surface-associated EPS (extracellular polymeric substances) and pooled in one test tube (5 ml, Eppendorf #0030119401).
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